CK Sequential Integration Series II-IV-A

Mentha × piperita

Peppermint

Tier IICK Sequential Integration Series

Target Trait

1.85×

fold-change

Cooling+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-05182

Genomic Edits

GC Content

43.8%

Construct Length

3,636 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + CaSR

IFI Score

185

Pathway Edits

TRPM8 pathway primary gene cluster overexpression for cooling axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized C+K dual-axis co-expression
Metabolic flux partitioning between IFI-C and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Cooling-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for C+K simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and kokumi accumulation without competing pathway suppressionSequential construct stacking for CK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II CK dual-axis sequential integration platform for food ingredient applications requiring verified combined cooling+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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