IFI-M · CB Tissue-Specific Series

Specimen SS-F-M2-05008 // MULTI-AXIS · IFI-M

CB Tissue-Specific Series III-BL-A

Mentha × piperita

Peppermint

Tier IIICB Tissue-Specific Series

Target Trait

1.95×

fold-change

Cooling+Bitter-Attenuation tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-05008

Genomic Edits

7

GC Content

45.2%

Construct Length

3,889 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPM8 + TAS2R38 (suppression)

IFI Score

195

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized C+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized CB spatial patterning

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and bitter-attenuation accumulation without competing pathway suppressionTissue-specific dual expression for CB multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III CB dual-axis tissue-specific platform for food ingredient applications requiring verified combined cooling+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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