IFI-M · CB Sequential Integration Series

Specimen SS-F-M2-04878 // MULTI-AXIS · IFI-M

CB Sequential Integration Series II-BL-C

Mentha × piperita

Peppermint

Tier IICB Sequential Integration Series

Target Trait

2.31×

fold-change

Cooling+Bitter-Attenuation sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-04878

Genomic Edits

8

GC Content

45.3%

Construct Length

4,428 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPM8 + TAS2R38 (suppression)

IFI Score

231

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized C+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+B simultaneous accumulation
  • Cofactor balance optimization for C+B dual-pathway operation
  • Protein scaffold engineering for C+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and bitter-attenuation accumulation without competing pathway suppressionSequential construct stacking for CB multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II CB dual-axis sequential integration platform for food ingredient applications requiring verified combined cooling+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$849 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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