CS Flux-Balanced Series II-AG-A

Mentha × piperita

Peppermint

Tier IICS Flux-Balanced Series

Target Trait

2.05×

fold-change

Cooling+Sweet flux-balanced output

Technical Specifications

SKU

SS-F-M2-04633

Genomic Edits

GC Content

47.5%

Construct Length

3,883 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPM8 + TAS1R2/TAS1R3

IFI Score

205

Pathway Edits

TRPM8 pathway primary gene cluster overexpression for cooling axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized C+S dual-axis co-expression
Metabolic flux partitioning between IFI-C and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Cooling-Sweet combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for C+S simultaneous accumulation
Cofactor balance optimization for C+S dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and sweet accumulation without competing pathway suppressionFlux-balanced gene dosing for CS multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II CS dual-axis flux-balanced platform for food ingredient applications requiring verified combined cooling+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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