IFI-M · CS Sequential Integration Series

Specimen SS-F-M2-04577 // MULTI-AXIS · IFI-M

CS Sequential Integration Series II-BL-B

Mentha × piperita

Peppermint

Tier IICS Sequential Integration Series

Target Trait

2.07×

fold-change

Cooling+Sweet sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-04577

Genomic Edits

7

GC Content

48.2%

Construct Length

3,931 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPM8 + TAS1R2/TAS1R3

IFI Score

207

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • Bidirectional promoter system for synchronized C+S dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-S pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Sweet combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+S simultaneous accumulation
  • Cofactor balance optimization for C+S dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and sweet accumulation without competing pathway suppressionSequential construct stacking for CS multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II CS dual-axis sequential integration platform for food ingredient applications requiring verified combined cooling+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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