IFI-M · CA Tissue-Specific Series

Specimen SS-F-M2-04436 // MULTI-AXIS · IFI-M

CA Tissue-Specific Series V-PR-B

Mentha × piperita

Peppermint

Tier VCA Tissue-Specific Series

Target Trait

3.14×

fold-change

Cooling+Astringency tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-04436

Genomic Edits

10

GC Content

44.4%

Construct Length

5,167 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPM8 + Polyphenol-astringency axis

IFI Score

314

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized C+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+A simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized CA spatial patterning
  • Protein scaffold engineering for C+A enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and astringency accumulation without competing pathway suppressionTissue-specific dual expression for CA multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V CA dual-axis tissue-specific platform for food ingredient applications requiring verified combined cooling+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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