IFI-M · CA Sequential Integration Series

Specimen SS-F-M2-04306 // MULTI-AXIS · IFI-M

CA Sequential Integration Series IV-IV-A

Mentha × piperita

Peppermint

Tier IVCA Sequential Integration Series

Target Trait

2.95×

fold-change

Cooling+Astringency sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-04306

Genomic Edits

9

GC Content

45.6%

Construct Length

4,746 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + Polyphenol-astringency axis

IFI Score

295

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized C+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+A simultaneous accumulation
  • Cofactor balance optimization for C+A dual-pathway operation
  • Protein scaffold engineering for C+A enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and astringency accumulation without competing pathway suppressionSequential construct stacking for CA multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV CA dual-axis sequential integration platform for food ingredient applications requiring verified combined cooling+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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