CU Flux-Balanced Series I-IV-B

Mentha × piperita

Peppermint

Tier ICU Flux-Balanced Series

Target Trait

1.57×

fold-change

Cooling+Umami flux-balanced output

Technical Specifications

SKU

SS-F-M2-04031

Genomic Edits

GC Content

46.2%

Construct Length

3,638 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + mGluR1

IFI Score

157

Pathway Edits

TRPM8 pathway primary gene cluster overexpression for cooling axis activation
mGluR1 pathway primary gene cluster overexpression for umami axis activation
Bidirectional promoter system for synchronized C+U dual-axis co-expression
Metabolic flux partitioning between IFI-C and IFI-U pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Cooling-Umami combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for C+U simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and umami accumulation without competing pathway suppressionFlux-balanced gene dosing for CU multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I CU dual-axis flux-balanced platform for food ingredient applications requiring verified combined cooling+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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