PK Tissue-Specific Series IV-IV-A

Brassica nigra

Black Mustard

Tier IVPK Tissue-Specific Series

Target Trait

2.38×

fold-change

Sharp+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-03826

Genomic Edits

GC Content

45.6%

Construct Length

4,406 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + CaSR

IFI Score

238

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized P+K dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+K simultaneous accumulation
Tissue-specific expression cassette deployment for optimized PK spatial patterning
Protein scaffold engineering for P+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for PK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PK dual-axis tissue-specific platform for food ingredient applications requiring verified combined sharp+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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