PK Tissue-Specific Series I-PR-A

Brassica nigra

Black Mustard

Tier IPK Tissue-Specific Series

Target Trait

1.1×

fold-change

Sharp+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-03787

Genomic Edits

GC Content

47%

Construct Length

2,730 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + CaSR

IFI Score

110

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized P+K dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-K pathway branches via carbon flow optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for PK multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PK dual-axis tissue-specific platform for food ingredient applications requiring verified combined sharp+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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