PK Sequential Integration Series IV-BL-A

Brassica nigra

Black Mustard

Tier IVPK Sequential Integration Series

Target Trait

2.95×

fold-change

Sharp+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-03700

Genomic Edits

GC Content

45.1%

Construct Length

4,644 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + CaSR

IFI Score

295

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized P+K dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+K simultaneous accumulation
Cofactor balance optimization for P+K dual-pathway operation
Protein scaffold engineering for P+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and kokumi accumulation without competing pathway suppressionSequential construct stacking for PK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PK dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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