PK Co-Expression Series I-IV-A

Brassica nigra

Black Mustard

Tier IPK Co-Expression Series

Target Trait

1.2×

fold-change

Sharp+Kokumi co-activation fold-change

Technical Specifications

SKU

SS-F-M2-03610

Genomic Edits

GC Content

43.5%

Construct Length

2,721 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + CaSR

IFI Score

120

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized P+K dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-K pathway branches via carbon flow optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and kokumi accumulation without competing pathway suppressionCo-expression vector design for PK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PK dual-axis co-expression platform for food ingredient applications requiring verified combined sharp+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Multi-Axis · IFI-M All Modalities