IFI-M · PB Precision Dual-Axis Series

Specimen SS-F-M2-03576 // MULTI-AXIS · IFI-M

PB Precision Dual-Axis Series III-IV-C

Brassica nigra

Black Mustard

Tier IIIPB Precision Dual-Axis Series

Target Trait

3.75×

fold-change

Sharp+Bitter-Attenuation precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-03576

Genomic Edits

11

GC Content

43.7%

Construct Length

5,465 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + TAS2R38 (suppression)

IFI Score

375

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized P+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PB spatial patterning
  • Chassis organism metabolic engineering for PB dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and bitter-attenuation accumulation without competing pathway suppressionPrecision combinatorial regulation for PB multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III PB dual-axis precision dual platform for food ingredient applications requiring verified combined sharp+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1649 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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