Specimen SS-F-M2-03531 // MULTI-AXIS · IFI-M
PB Tissue-Specific Series V-AG-C
Brassica nigra
Black Mustard
Target Trait
3.5×
fold-change
Sharp+Bitter-Attenuation tissue-specific dual expression
Technical Specifications
SKU
SS-F-M2-03531
Genomic Edits
11
GC Content
49.1%
Construct Length
5,562 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TRPA1 + TAS2R38 (suppression)
IFI Score
350
Pathway Edits
- TRPA1 pathway primary gene cluster overexpression for sharp axis activation
- TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
- Bidirectional promoter system for synchronized P+B dual-axis co-expression
- Metabolic flux partitioning between IFI-P and IFI-B pathway branches via carbon flow optimization
- Transcription factor co-binding site engineering for Sharp-Bitter-Attenuation combinatorial gene regulation
- Cross-pathway feedback inhibition circuit removal for P+B simultaneous accumulation
- Tissue-specific expression cassette deployment for optimized PB spatial patterning
- Protein scaffold engineering for P+B enzyme co-localization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V PB dual-axis tissue-specific platform for food ingredient applications requiring verified combined sharp+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.
License
$10149 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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