Specimen SS-F-M2-03404 // MULTI-AXIS · IFI-M
PB Sequential Integration Series IV-PR-B
Brassica nigra
Black Mustard
Target Trait
3.3×
fold-change
Sharp+Bitter-Attenuation sequential pathway fold-change
Technical Specifications
SKU
SS-F-M2-03404
Genomic Edits
10
GC Content
43.9%
Construct Length
5,192 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-mediated
Receptor Target
TRPA1 + TAS2R38 (suppression)
IFI Score
330
Pathway Edits
- TRPA1 pathway primary gene cluster overexpression for sharp axis activation
- TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
- Bidirectional promoter system for synchronized P+B dual-axis co-expression
- Metabolic flux partitioning between IFI-P and IFI-B pathway branches via carbon flow optimization
- Transcription factor co-binding site engineering for Sharp-Bitter-Attenuation combinatorial gene regulation
- Cross-pathway feedback inhibition circuit removal for P+B simultaneous accumulation
- Cofactor balance optimization for P+B dual-pathway operation
- Protein scaffold engineering for P+B enzyme co-localization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV PB dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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