PB Co-Expression Series IV-BL-C

Brassica nigra

Black Mustard

Tier IVPB Co-Expression Series

Target Trait

3.19×

fold-change

Sharp+Bitter-Attenuation co-activation fold-change

Technical Specifications

SKU

SS-F-M2-03342

Genomic Edits

GC Content

43.8%

Construct Length

5,138 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + TAS2R38 (suppression)

IFI Score

319

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
Bidirectional promoter system for synchronized P+B dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-B pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Bitter-Attenuation combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+B simultaneous accumulation
Cofactor balance optimization for P+B dual-pathway operation
Protein scaffold engineering for P+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and bitter-attenuation accumulation without competing pathway suppressionCo-expression vector design for PB multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PB dual-axis co-expression platform for food ingredient applications requiring verified combined sharp+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4149 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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