IFI-M · PS Tissue-Specific Series

Specimen SS-F-M2-03233 // MULTI-AXIS · IFI-M

PS Tissue-Specific Series V-BL-B

Brassica nigra

Black Mustard

Tier VPS Tissue-Specific Series

Target Trait

3.14×

fold-change

Sharp+Sweet tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-03233

Genomic Edits

10

GC Content

47.5%

Construct Length

5,116 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + TAS1R2/TAS1R3

IFI Score

314

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • Bidirectional promoter system for synchronized P+S dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-S pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Sweet combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+S simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PS spatial patterning
  • Protein scaffold engineering for P+S enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and sweet accumulation without competing pathway suppressionTissue-specific dual expression for PS multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V PS dual-axis tissue-specific platform for food ingredient applications requiring verified combined sharp+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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