PS Sequential Integration Series III-BL-C

Brassica nigra

Black Mustard

Tier IIIPS Sequential Integration Series

Target Trait

3×

fold-change

Sharp+Sweet sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-03090

Genomic Edits

GC Content

43.5%

Construct Length

5,023 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + TAS1R2/TAS1R3

IFI Score

300

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized P+S dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Sweet combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+S simultaneous accumulation
Cofactor balance optimization for P+S dual-pathway operation
Protein scaffold engineering for P+S enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and sweet accumulation without competing pathway suppressionSequential construct stacking for PS multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III PS dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1649 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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