PS Sequential Integration Series I-IV-B

Brassica nigra

Black Mustard

Tier IPS Sequential Integration Series

Target Trait

1.46×

fold-change

Sharp+Sweet sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-03071

Genomic Edits

GC Content

46.2%

Construct Length

3,518 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + TAS1R2/TAS1R3

IFI Score

146

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized P+S dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Sweet combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+S simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and sweet accumulation without competing pathway suppressionSequential construct stacking for PS multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PS dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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