PA Precision Dual-Axis Series I-IV-A

Brassica nigra

Black Mustard

Tier IPA Precision Dual-Axis Series

Target Trait

1.5×

fold-change

Sharp+Astringency precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-02950

Genomic Edits

GC Content

44.8%

Construct Length

3,661 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + Polyphenol-astringency axis

IFI Score

150

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
Bidirectional promoter system for synchronized P+A dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-A pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Astringency combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+A simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and astringency accumulation without competing pathway suppressionPrecision combinatorial regulation for PA multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PA dual-axis precision dual platform for food ingredient applications requiring verified combined sharp+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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