IFI-M · PA Tissue-Specific Series

Specimen SS-F-M2-02939 // MULTI-AXIS · IFI-M

PA Tissue-Specific Series V-IV-B

Brassica nigra

Black Mustard

Tier VPA Tissue-Specific Series

Target Trait

3.14×

fold-change

Sharp+Astringency tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-02939

Genomic Edits

10

GC Content

46.5%

Construct Length

5,118 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + Polyphenol-astringency axis

IFI Score

314

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized P+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+A simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PA spatial patterning
  • Protein scaffold engineering for P+A enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and astringency accumulation without competing pathway suppressionTissue-specific dual expression for PA multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V PA dual-axis tissue-specific platform for food ingredient applications requiring verified combined sharp+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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