IFI-M · PA Flux-Balanced Series

Specimen SS-F-M2-02832 // MULTI-AXIS · IFI-M

PA Flux-Balanced Series I-IV-C

Brassica nigra

Black Mustard

Tier IPA Flux-Balanced Series

Target Trait

1.75×

fold-change

Sharp+Astringency flux-balanced output

Technical Specifications

SKU

SS-F-M2-02832

Genomic Edits

7

GC Content

45.9%

Construct Length

4,035 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + Polyphenol-astringency axis

IFI Score

175

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized P+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+A simultaneous accumulation
  • Cofactor balance optimization for P+A dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and astringency accumulation without competing pathway suppressionFlux-balanced gene dosing for PA multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I PA dual-axis flux-balanced platform for food ingredient applications requiring verified combined sharp+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$449 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Multi-Axis · IFI-MAll Modalities