IFI-M · PA Sequential Integration Series

Specimen SS-F-M2-02801 // MULTI-AXIS · IFI-M

PA Sequential Integration Series IV-BL-B

Brassica nigra

Black Mustard

Tier IVPA Sequential Integration Series

Target Trait

3.3×

fold-change

Sharp+Astringency sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-02801

Genomic Edits

10

GC Content

45.8%

Construct Length

5,141 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + Polyphenol-astringency axis

IFI Score

330

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized P+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+A simultaneous accumulation
  • Cofactor balance optimization for P+A dual-pathway operation
  • Protein scaffold engineering for P+A enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and astringency accumulation without competing pathway suppressionSequential construct stacking for PA multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV PA dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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