IFI-M · PU Precision Dual-Axis Series

Specimen SS-F-M2-02686 // MULTI-AXIS · IFI-M

PU Precision Dual-Axis Series IV-IV-A

Brassica nigra

Black Mustard

Tier IVPU Precision Dual-Axis Series

Target Trait

3.75×

fold-change

Sharp+Umami precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-02686

Genomic Edits

11

GC Content

44.7%

Construct Length

5,566 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + mGluR1

IFI Score

375

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • Bidirectional promoter system for synchronized P+U dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-U pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Umami combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+U simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PU spatial patterning
  • Chassis organism metabolic engineering for PU dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and umami accumulation without competing pathway suppressionPrecision combinatorial regulation for PU multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV PU dual-axis precision dual platform for food ingredient applications requiring verified combined sharp+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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