IFI-M · PU Precision Dual-Axis Series

Specimen SS-F-M2-02643 // MULTI-AXIS · IFI-M

PU Precision Dual-Axis Series I-AG-C

Brassica nigra

Black Mustard

Tier IPU Precision Dual-Axis Series

Target Trait

1.88×

fold-change

Sharp+Umami precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-02643

Genomic Edits

8

GC Content

47.1%

Construct Length

4,402 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPA1 + mGluR1

IFI Score

188

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • Bidirectional promoter system for synchronized P+U dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-U pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Umami combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+U simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PU spatial patterning
  • Chassis organism metabolic engineering for PU dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and umami accumulation without competing pathway suppressionPrecision combinatorial regulation for PU multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I PU dual-axis precision dual platform for food ingredient applications requiring verified combined sharp+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$449 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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