PU Flux-Balanced Series III-IV-A

Brassica nigra

Black Mustard

Tier IIIPU Flux-Balanced Series

Target Trait

2.7×

fold-change

Sharp+Umami flux-balanced output

Technical Specifications

SKU

SS-F-M2-02554

Genomic Edits

GC Content

43.6%

Construct Length

4,351 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + mGluR1

IFI Score

270

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
mGluR1 pathway primary gene cluster overexpression for umami axis activation
Bidirectional promoter system for synchronized P+U dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-U pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Umami combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+U simultaneous accumulation
Cofactor balance optimization for P+U dual-pathway operation
Protein scaffold engineering for P+U enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and umami accumulation without competing pathway suppressionFlux-balanced gene dosing for PU multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III PU dual-axis flux-balanced platform for food ingredient applications requiring verified combined sharp+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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