PU Sequential Integration Series I-PR-B

Brassica nigra

Black Mustard

Tier IPU Sequential Integration Series

Target Trait

1.46×

fold-change

Sharp+Umami sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-02468

Genomic Edits

GC Content

43.4%

Construct Length

3,667 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + mGluR1

IFI Score

146

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
mGluR1 pathway primary gene cluster overexpression for umami axis activation
Bidirectional promoter system for synchronized P+U dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-U pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Umami combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+U simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and umami accumulation without competing pathway suppressionSequential construct stacking for PU multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PU dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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