IFI-M · PC Precision Dual-Axis Series

Specimen SS-F-M2-02375 // MULTI-AXIS · IFI-M

PC Precision Dual-Axis Series III-IV-B

Brassica nigra

Black Mustard

Tier IIIPC Precision Dual-Axis Series

Target Trait

3.36×

fold-change

Sharp+Cooling precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-02375

Genomic Edits

10

GC Content

47.3%

Construct Length

5,068 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + TRPM8

IFI Score

336

Pathway Edits

  • TRPA1 pathway primary gene cluster overexpression for sharp axis activation
  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • Bidirectional promoter system for synchronized P+C dual-axis co-expression
  • Metabolic flux partitioning between IFI-P and IFI-C pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sharp-Cooling combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for P+C simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized PC spatial patterning
  • Chassis organism metabolic engineering for PC dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and cooling accumulation without competing pathway suppressionPrecision combinatorial regulation for PC multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III PC dual-axis precision dual platform for food ingredient applications requiring verified combined sharp+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Multi-Axis · IFI-MAll Modalities