PC Sequential Integration Series II-PR-A

Brassica nigra

Black Mustard

Tier IIPC Sequential Integration Series

Target Trait

1.85×

fold-change

Sharp+Cooling sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-02179

Genomic Edits

GC Content

46.5%

Construct Length

3,585 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + TRPM8

IFI Score

185

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TRPM8 pathway primary gene cluster overexpression for cooling axis activation
Bidirectional promoter system for synchronized P+C dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-C pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Cooling combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+C simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and cooling accumulation without competing pathway suppressionSequential construct stacking for PC multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II PC dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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