PC Sequential Integration Series I-BL-B

Brassica nigra

Black Mustard

Tier IPC Sequential Integration Series

Target Trait

1.46×

fold-change

Sharp+Cooling sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-02165

Genomic Edits

GC Content

48.5%

Construct Length

3,516 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + TRPM8

IFI Score

146

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TRPM8 pathway primary gene cluster overexpression for cooling axis activation
Bidirectional promoter system for synchronized P+C dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-C pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sharp-Cooling combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for P+C simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and cooling accumulation without competing pathway suppressionSequential construct stacking for PC multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PC dual-axis sequential integration platform for food ingredient applications requiring verified combined sharp+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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