PC Co-Expression Series I-BL-A

Brassica nigra

Black Mustard

Tier IPC Co-Expression Series

Target Trait

1.2×

fold-change

Sharp+Cooling co-activation fold-change

Technical Specifications

SKU

SS-F-M2-02104

Genomic Edits

GC Content

44.3%

Construct Length

2,919 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + TRPM8

IFI Score

120

Pathway Edits

TRPA1 pathway primary gene cluster overexpression for sharp axis activation
TRPM8 pathway primary gene cluster overexpression for cooling axis activation
Bidirectional promoter system for synchronized P+C dual-axis co-expression
Metabolic flux partitioning between IFI-P and IFI-C pathway branches via carbon flow optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPA1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sharp and cooling accumulation without competing pathway suppressionCo-expression vector design for PC multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PC dual-axis co-expression platform for food ingredient applications requiring verified combined sharp+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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