IFI-M · HK Tissue-Specific Series

Specimen SS-F-M2-02040 // MULTI-AXIS · IFI-M

HK Tissue-Specific Series V-IV-C

Capsicum chinense

Habanero

Tier VHK Tissue-Specific Series

Target Trait

3.5×

fold-change

Heat+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-02040

Genomic Edits

11

GC Content

44.2%

Construct Length

5,415 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + CaSR

IFI Score

350

Pathway Edits

  • TRPV1 pathway primary gene cluster overexpression for heat axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized H+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-H and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Heat-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for H+K simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized HK spatial patterning
  • Protein scaffold engineering for H+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for HK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V HK dual-axis tissue-specific platform for food ingredient applications requiring verified combined heat+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10149 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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