HK Sequential Integration Series I-AG-C

Capsicum chinense

Habanero

Tier IHK Sequential Integration Series

Target Trait

1.63×

fold-change

Heat+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-01863

Genomic Edits

GC Content

48.9%

Construct Length

3,962 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPV1 + CaSR

IFI Score

163

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized H+K dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+K simultaneous accumulation
Cofactor balance optimization for H+K dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and kokumi accumulation without competing pathway suppressionSequential construct stacking for HK multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I HK dual-axis sequential integration platform for food ingredient applications requiring verified combined heat+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$449 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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