IFI-M · HB Tissue-Specific Series

Specimen SS-F-M2-01719 // MULTI-AXIS · IFI-M

HB Tissue-Specific Series IV-AG-C

Capsicum chinense

Habanero

Tier IVHB Tissue-Specific Series

Target Trait

2.97×

fold-change

Heat+Bitter-Attenuation tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-01719

Genomic Edits

10

GC Content

47.4%

Construct Length

5,147 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPV1 + TAS2R38 (suppression)

IFI Score

297

Pathway Edits

  • TRPV1 pathway primary gene cluster overexpression for heat axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized H+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-H and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Heat-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for H+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized HB spatial patterning
  • Protein scaffold engineering for H+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and bitter-attenuation accumulation without competing pathway suppressionTissue-specific dual expression for HB multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV HB dual-axis tissue-specific platform for food ingredient applications requiring verified combined heat+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4149 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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