IFI-M · HB Sequential Integration Series

Specimen SS-F-M2-01608 // MULTI-AXIS · IFI-M

HB Sequential Integration Series IV-IV-C

Capsicum chinense

Habanero

Tier IVHB Sequential Integration Series

Target Trait

3.69×

fold-change

Heat+Bitter-Attenuation sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-01608

Genomic Edits

11

GC Content

45.2%

Construct Length

5,440 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + TAS2R38 (suppression)

IFI Score

369

Pathway Edits

  • TRPV1 pathway primary gene cluster overexpression for heat axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized H+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-H and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Heat-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for H+B simultaneous accumulation
  • Cofactor balance optimization for H+B dual-pathway operation
  • Protein scaffold engineering for H+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and bitter-attenuation accumulation without competing pathway suppressionSequential construct stacking for HB multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV HB dual-axis sequential integration platform for food ingredient applications requiring verified combined heat+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4149 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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