HS Flux-Balanced Series I-IV-B

Capsicum chinense

Habanero

Tier IHS Flux-Balanced Series

Target Trait

1.57×

fold-change

Heat+Sweet flux-balanced output

Technical Specifications

SKU

SS-F-M2-01331

Genomic Edits

GC Content

49.1%

Construct Length

3,538 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + TAS1R2/TAS1R3

IFI Score

157

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized H+S dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Sweet combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+S simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and sweet accumulation without competing pathway suppressionFlux-balanced gene dosing for HS multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I HS dual-axis flux-balanced platform for food ingredient applications requiring verified combined heat+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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