HA Flux-Balanced Series I-AG-B

Capsicum chinense

Habanero

Tier IHA Flux-Balanced Series

Target Trait

1.57×

fold-change

Heat+Astringency flux-balanced output

Technical Specifications

SKU

SS-F-M2-01022

Genomic Edits

GC Content

43.8%

Construct Length

3,485 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPV1 + Polyphenol-astringency axis

IFI Score

157

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
Bidirectional promoter system for synchronized H+A dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-A pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Astringency combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+A simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and astringency accumulation without competing pathway suppressionFlux-balanced gene dosing for HA multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I HA dual-axis flux-balanced platform for food ingredient applications requiring verified combined heat+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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