HU Tissue-Specific Series III-IV-A

Capsicum chinense

Habanero

Tier IIIHU Tissue-Specific Series

Target Trait

1.95×

fold-change

Heat+Umami tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-00814

Genomic Edits

GC Content

45.4%

Construct Length

3,991 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + mGluR1

IFI Score

195

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
mGluR1 pathway primary gene cluster overexpression for umami axis activation
Bidirectional promoter system for synchronized H+U dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-U pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Umami combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+U simultaneous accumulation
Tissue-specific expression cassette deployment for optimized HU spatial patterning

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and umami accumulation without competing pathway suppressionTissue-specific dual expression for HU multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III HU dual-axis tissue-specific platform for food ingredient applications requiring verified combined heat+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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