HU Co-Expression Series II-IV-A

Capsicum chinense

Habanero

Tier IIHU Co-Expression Series

Target Trait

1.65×

fold-change

Heat+Umami co-activation fold-change

Technical Specifications

SKU

SS-F-M2-00622

Genomic Edits

GC Content

43.8%

Construct Length

3,136 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + mGluR1

IFI Score

165

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
mGluR1 pathway primary gene cluster overexpression for umami axis activation
Bidirectional promoter system for synchronized H+U dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-U pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Umami combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and mGluR1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and umami accumulation without competing pathway suppressionCo-expression vector design for HU multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II HU dual-axis co-expression platform for food ingredient applications requiring verified combined heat+umami sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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