HC Tissue-Specific Series IV-BL-C

Capsicum chinense

Habanero

Tier IVHC Tissue-Specific Series

Target Trait

2.97×

fold-change

Heat+Cooling tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-00522

Genomic Edits

GC Content

46.3%

Construct Length

5,198 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPV1 + TRPM8

IFI Score

297

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
TRPM8 pathway primary gene cluster overexpression for cooling axis activation
Bidirectional promoter system for synchronized H+C dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-C pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Cooling combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+C simultaneous accumulation
Tissue-specific expression cassette deployment for optimized HC spatial patterning
Protein scaffold engineering for H+C enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and cooling accumulation without competing pathway suppressionTissue-specific dual expression for HC multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV HC dual-axis tissue-specific platform for food ingredient applications requiring verified combined heat+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4149 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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