HC Co-Expression Series II-PR-A

Capsicum chinense

Habanero

Tier IIHC Co-Expression Series

Target Trait

1.65×

fold-change

Heat+Cooling co-activation fold-change

Technical Specifications

SKU

SS-F-M2-00319

Genomic Edits

GC Content

47.4%

Construct Length

3,185 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPV1 + TRPM8

IFI Score

165

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
TRPM8 pathway primary gene cluster overexpression for cooling axis activation
Bidirectional promoter system for synchronized H+C dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-C pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Cooling combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TRPM8 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and cooling accumulation without competing pathway suppressionCo-expression vector design for HC multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II HC dual-axis co-expression platform for food ingredient applications requiring verified combined heat+cooling sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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