HP Tissue-Specific Series II-IV-C

Capsicum chinense

Habanero

Tier IIHP Tissue-Specific Series

Target Trait

1.9×

fold-change

Heat+Sharp tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-00204

Genomic Edits

GC Content

43.9%

Construct Length

3,990 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + TRPA1

IFI Score

190

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
TRPA1 pathway primary gene cluster overexpression for sharp axis activation
Bidirectional promoter system for synchronized H+P dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-P pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Sharp combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+P simultaneous accumulation
Tissue-specific expression cassette deployment for optimized HP spatial patterning

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TRPA1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and sharp accumulation without competing pathway suppressionTissue-specific dual expression for HP multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II HP dual-axis tissue-specific platform for food ingredient applications requiring verified combined heat+sharp sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$849 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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