HP Flux-Balanced Series III-IV-B

Capsicum chinense

Habanero

Tier IIIHP Flux-Balanced Series

Target Trait

3.02×

fold-change

Heat+Sharp flux-balanced output

Technical Specifications

SKU

SS-F-M2-00155

Genomic Edits

GC Content

48.7%

Construct Length

4,748 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPV1 + TRPA1

IFI Score

302

Pathway Edits

TRPV1 pathway primary gene cluster overexpression for heat axis activation
TRPA1 pathway primary gene cluster overexpression for sharp axis activation
Bidirectional promoter system for synchronized H+P dual-axis co-expression
Metabolic flux partitioning between IFI-H and IFI-P pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Heat-Sharp combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for H+P simultaneous accumulation
Cofactor balance optimization for H+P dual-pathway operation
Protein scaffold engineering for H+P enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPV1 and TRPA1 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous heat and sharp accumulation without competing pathway suppressionFlux-balanced gene dosing for HP multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III HP dual-axis flux-balanced platform for food ingredient applications requiring verified combined heat+sharp sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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