Kokumi-Rich Allium Total Engineering V-PR-B

Allium sativum

Garlic

Tier VKokumi-Rich Allium Total Engineering

Target Trait

6.48×

fold-change

Comprehensive kokumi compound co-production fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-998

Genomic Edits

GC Content

46.9%

Construct Length

6,261 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-4000

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

648

Pathway Edits

GCL/GS comprehensive GSH pathway co-engineering for primary kokumi compound foundation
GAD1 high-flux GABA production cassette for CaSR allosteric modulator co-production
SPDS spermidine synthesis activation for polyamine CaSR ligand co-engineering
ACSO-lyase SAC production for S-allyl-cysteine CaSR agonist co-expression
SAT cysteine sulfoxide channeling for alliin and SAC precursor flux optimization
GGT1 γ-glutamyl transpeptidation for dipeptide and tripeptide CaSR ligand decoration
Multi-compound pathway flux balancing via transcriptional co-regulator expression
Metabolic hub optimization for simultaneous GSH, GABA, spermidine and SAC production

Key Interventions

GCL/GS/GAD1/SPDS comprehensive four-compound kokumi co-production for full-spectrum CaSR occupancyACSO-lyase/SAT SAC and alliin CaSR ligand co-engineering for sulfur agonist spectrum amplificationGGT1 γ-glutamyl transpeptidation for maximal broad-spectrum CaSR occupancy across all ligand classesProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 13 precision edits for 6.48× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

648

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Ultra-premium total Allium kokumi pinnacle for luxury garlic-forward brand product differentiation

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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