Kokumi-Rich Allium Total Engineering IV-IV-A

Allium sativum

Garlic

Tier IVKokumi-Rich Allium Total Engineering

Target Trait

5×

fold-change

Comprehensive kokumi compound co-production fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-991

Genomic Edits

GC Content

44.8%

Construct Length

5,551 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

500

Pathway Edits

GCL/GS comprehensive GSH pathway co-engineering for primary kokumi compound foundation
GAD1 high-flux GABA production cassette for CaSR allosteric modulator co-production
SPDS spermidine synthesis activation for polyamine CaSR ligand co-engineering
ACSO-lyase SAC production for S-allyl-cysteine CaSR agonist co-expression
SAT cysteine sulfoxide channeling for alliin and SAC precursor flux optimization
GGT1 γ-glutamyl transpeptidation for dipeptide and tripeptide CaSR ligand decoration
Multi-compound pathway flux balancing via transcriptional co-regulator expression
Metabolic hub optimization for simultaneous GSH, GABA, spermidine and SAC production

Key Interventions

GCL/GS/GAD1/SPDS comprehensive four-compound kokumi co-production for full-spectrum CaSR occupancyACSO-lyase/SAT SAC and alliin CaSR ligand co-engineering for sulfur agonist spectrum amplificationGGT1 γ-glutamyl transpeptidation for maximal broad-spectrum CaSR occupancy across all ligand classesCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 11 precision edits for 5× fold-change

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

500

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Premium total Allium kokumi ingredient for restaurant flagship garlic and savory applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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