IFI-K · Kokumi-Rich Allium Total Engineering
Specimen SS-F-KOK-976 // KOKUMI · IFI-K
Kokumi-Rich Allium Total Engineering II-IV-B
Allium sativum
Garlic
Tier IIKokumi-Rich Allium Total Engineering
Target Trait
3.24×
fold-change
Comprehensive kokumi compound co-production fold-change in Allium sativum
Technical Specifications
SKU
SS-F-KOK-976
Genomic Edits
9
GC Content
47.3%
Construct Length
4,674 bp
Promoter System
T7/SP6 cell-free
Expression System
Cell-free in vitro transcription/translation
Host Strain
T7 RNA Polymerase
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
324
Pathway Edits
- GCL/GS comprehensive GSH pathway co-engineering for primary kokumi compound foundation
- GAD1 high-flux GABA production cassette for CaSR allosteric modulator co-production
- SPDS spermidine synthesis activation for polyamine CaSR ligand co-engineering
- ACSO-lyase SAC production for S-allyl-cysteine CaSR agonist co-expression
- SAT cysteine sulfoxide channeling for alliin and SAC precursor flux optimization
- GGT1 γ-glutamyl transpeptidation for dipeptide and tripeptide CaSR ligand decoration
- Multi-compound pathway flux balancing via transcriptional co-regulator expression
- Metabolic hub optimization for simultaneous GSH, GABA, spermidine and SAC production
Key Interventions
GCL/GS/GAD1/SPDS comprehensive four-compound kokumi co-production for full-spectrum CaSR occupancyACSO-lyase/SAT SAC and alliin CaSR ligand co-engineering for sulfur agonist spectrum amplificationGGT1 γ-glutamyl transpeptidation for maximal broad-spectrum CaSR occupancy across all ligand classesCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-II construct validated with 9 precision edits for 3.24× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K324
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Total Allium kokumi foundation for pilot premium garlic-forward broth and sauce development
License
$749 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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