IFI-K · Propionamide Analog

Specimen SS-F-KOK-951 // KOKUMI · IFI-K

Propionamide Analog IV-IV-A

Saccharomyces cerevisiae

Yeast

Tier IVPropionamide Analog

Target Trait

2.38×

fold-change

Glutathione-analog amide CaSR kokumi fold-change in Saccharomyces cerevisiae

Technical Specifications

SKU

SS-F-KOK-951

Genomic Edits

5

GC Content

44.8%

Construct Length

3,191 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

238

Pathway Edits

  • BAHD-AT (BAHD acyltransferase) expression for propionyl group transfer to GSH scaffold
  • GCL substrate supply for γ-glutamyl-cysteinyl scaffold production as BAHD-AT acceptor
  • GS-alt (alternative glutathione synthetase) expression for amide bond formation on analog scaffold
  • AAT (amino acid acetyltransferase) expression for amide bond optimization in analog structure
  • Amide-GSH analog permease expression for cytosolic accumulation of CaSR-active analog

Key Interventions

BAHD-AT acyltransferase expression for propionamide-GSH analog biosynthesis as CaSR kokumi ligandGCL/GS-alt γ-glutamyl-cysteinyl scaffold supply for BAHD-AT-catalyzed acyl modificationAAT amide bond formation optimization for structurally constrained CaSR analog engagementCell-free in vitro transcription/translation delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-IV construct validated with 5 precision edits for 2.38× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
238
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium yeast-fermented propionamide analog for specialty culinary and artisan applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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