IFI-K · Propionamide Analog

Specimen SS-F-KOK-929 // KOKUMI · IFI-K

Propionamide Analog II-AG-A

Saccharomyces cerevisiae

Yeast

Tier IIPropionamide Analog

Target Trait

1.52×

fold-change

Glutathione-analog amide CaSR kokumi fold-change in Saccharomyces cerevisiae

Technical Specifications

SKU

SS-F-KOK-929

Genomic Edits

4

GC Content

45.2%

Construct Length

2,775 bp

Promoter System

Tissue-specific 2A11 / CaMV 35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

152

Pathway Edits

  • BAHD-AT (BAHD acyltransferase) expression for propionyl group transfer to GSH scaffold
  • GCL substrate supply for γ-glutamyl-cysteinyl scaffold production as BAHD-AT acceptor
  • GS-alt (alternative glutathione synthetase) expression for amide bond formation on analog scaffold
  • AAT (amino acid acetyltransferase) expression for amide bond optimization in analog structure
  • Amide-GSH analog permease expression for cytosolic accumulation of CaSR-active analog

Key Interventions

BAHD-AT acyltransferase expression for propionamide-GSH analog biosynthesis as CaSR kokumi ligandGCL/GS-alt γ-glutamyl-cysteinyl scaffold supply for BAHD-AT-catalyzed acyl modificationAAT amide bond formation optimization for structurally constrained CaSR analog engagementAgrobacterium-mediated T-DNA delivery optimized for Saccharomyces cerevisiae genome accessibilityTier-II construct validated with 4 precision edits for 1.52× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
152
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Yeast-platform propionamide kokumi foundation for pilot fermented ingredient development

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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