IFI-K · Glutamyl Transpeptidase Optimization
Specimen SS-F-KOK-918 // KOKUMI · IFI-K
Glutamyl Transpeptidase Optimization V-PR-B
Allium sativum
Garlic
Tier VGlutamyl Transpeptidase Optimization
Target Trait
3.78×
fold-change
GGT-driven free γ-glutamyl peptide amplification fold-change in Allium sativum
Technical Specifications
SKU
SS-F-KOK-918
Genomic Edits
8
GC Content
46.9%
Construct Length
4,401 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-4000
Receptor Target
CaSR (calcium-sensing receptor)
IFI Score
378
Pathway Edits
- GGT1 (γ-glutamyl transpeptidase 1) catalytic activity optimization via active-site engineering
- GGT4-high (high-expression GGT4 isoform) overexpression cassette for parallel transfer pathway
- GGT-VAC (vacuolar GGT isoform) expression for substrate mobilization from vacuolar GSH pool
- AA-perm (amino acid permease) upregulation for acceptor substrate import and GGT reaction rate
- Glutamate donor pool expansion via GS feedback-loop attenuation
- Transpeptidase specificity maturation for preferred acceptor amino acid bias toward CaSR ligands
Key Interventions
GGT1/GGT4-high dual-isoform overexpression for high-flux γ-glutamyl transpeptidation producing diverse CaSR ligandsGGT-VAC vacuolar isoform expression for mobilization of vacuolar GSH pool into free γ-glutamyl peptide productsAA-perm acceptor substrate import upregulation for maximum GGT reaction rate and product diversityProtoplast PEG-fusion delivery optimized for Allium sativum genome accessibilityTier-V construct validated with 8 precision edits for 3.78× fold-change
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K378
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Ultra-premium GGT-optimized γ-glutamyl peptide kokumi for luxury garlic product differentiation
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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