IFI-K · Glutamyl Transpeptidase Optimization

Specimen SS-F-KOK-912 // KOKUMI · IFI-K

Glutamyl Transpeptidase Optimization IV-IV-B

Allium sativum

Garlic

Tier IVGlutamyl Transpeptidase Optimization

Target Trait

3.22×

fold-change

GGT-driven free γ-glutamyl peptide amplification fold-change in Allium sativum

Technical Specifications

SKU

SS-F-KOK-912

Genomic Edits

7

GC Content

46.2%

Construct Length

3,888 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free in vitro transcription/translation

Host Strain

T7 RNA Polymerase

Receptor Target

CaSR (calcium-sensing receptor)

IFI Score

322

Pathway Edits

  • GGT1 (γ-glutamyl transpeptidase 1) catalytic activity optimization via active-site engineering
  • GGT4-high (high-expression GGT4 isoform) overexpression cassette for parallel transfer pathway
  • GGT-VAC (vacuolar GGT isoform) expression for substrate mobilization from vacuolar GSH pool
  • AA-perm (amino acid permease) upregulation for acceptor substrate import and GGT reaction rate
  • Glutamate donor pool expansion via GS feedback-loop attenuation
  • Transpeptidase specificity maturation for preferred acceptor amino acid bias toward CaSR ligands

Key Interventions

GGT1/GGT4-high dual-isoform overexpression for high-flux γ-glutamyl transpeptidation producing diverse CaSR ligandsGGT-VAC vacuolar isoform expression for mobilization of vacuolar GSH pool into free γ-glutamyl peptide productsAA-perm acceptor substrate import upregulation for maximum GGT reaction rate and product diversityCell-free in vitro transcription/translation delivery optimized for Allium sativum genome accessibilityTier-IV construct validated with 7 precision edits for 3.22× fold-change

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
322
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium GGT-driven garlic kokumi ingredient for restaurant and specialty food applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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